Ranjeet kumar RNTCP Ranjeet kumar added with RNTCP
DIAGNOSTIC TOOLS Tools for microbiological confirmation of TB All efforts should be undertaken for microbiologically confirming the diagnosis in presumptive TB patients. Under RNTCP, the acceptable methods for microbiological diagnosis of TB are: Sputum Smear Microscopy (for AFB): Zeihl-Neelson Staining Fluorescence staining Culture : Solid (Lowenstein Jensen) media Automated Liquid culture systems e.g. BACTEC MGIT960, BactiAlertorVersatreketc. Drug Sensitivity Testing: Modified PST for MGIT 960 system (for both first and second line drugs) Economic variant of Proportion sensitivity testing (1 %) using LJ medium (as a back up when indicated) Rapid molecular diagnostic testing: Line Probe Assay for MTB complex and detection of RIF& INH resistance NucleicAcid Amplification Test (NAAT) Xpert MTB/Rif testing using the GeneXpert system Smear microscopy being the most commonly used method for microbiological diagnosis of TB for the last several decades, has had en